Cotinine (COT) Rapid Test - Oral Fluid

Cotinine is the first-stage metabolite of nicotine, a toxic alkaloid that produces stimulation of the autonomic ganglia and central nervous system when in humans. Nicotine is a drug to which virtually every member of a tobacco-smoking society is exposed whether through direct contact or second-hand inhalation. In addition to tobacco, nicotine is also commercially available as the active ingredient in smoking replacement therapies such as nicotine gum, transdermal patches and nasal sprays.

The COT Rapid Test Cassette/Midstream (Oral fluid) yields a positive result when the cotinineconcentration in oral fluid exceeds 20 ng/mL.

【DIRECTIONS FOR CASSETTE USE】
Allow the test cassette, specimen, and/or controls to reach room temperature (15-30ºC) prior to testing. Instruct the donor to not place anything in the mouth including food, drink, gum or tobacco products for at least 10 minutes prior to collection.
1. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it within one hour.
2. Remove the collector from the sealed pouch and insert the sponge end of the collector into the mouth. Actively swab the inside of the mouth and tongue to collect oral fluid for a total of 3 minutes until the sponge becomes fully saturated. Gentle pressing the sponge between the tongue and teeth will assist saturation. No hard spots should be felt on the sponge when saturated.
3. Remove the collector from the mouth. Place saturated oral fluid collector into chamber and press sponge fully against the strainer to collect oral fluid. Discard the collector. Snap the cap shut on the collection tube.
4. Place the test cassette on a clean and level surface. Unscrew cap cover from the collection tube. Invert the collection tube and transfer 3 drops of oral fluid (approximately 120 µL) into specimen well of the test cassette and start the timer. Avoid trapping air bubbles in the specimen well. Place screw cap on the collection tube. See illustration below.
5. Wait for the colored line(s) to appear. Read results at 10 minutes. Do not read results after 1 hour.

【DIRECTIONS FOR DEVICE USE】

Allow the test device, and/or controls to reach room temperature (15-30°C) prior to testing. Advise the donor not to have anything in the mouth including food, drink, gum or tobacco etc for10 minutes at least prior to saliva collection.

1. Remove the test device from the sealed pouch and use it within one hour.

2. Insert the absorbent wick to the mouth and put it under the tongue to collect oral fluid until the control line appears.

3. Place the test device on a clean and level surface. See illustration below.

4. Read results at 10 minutes. Do not read results after 15 minutes.